NDLI: Combining Voltage and Calcium Imaging from Neuronal Dendrites

Content Provider	Springer Nature Link
Author	Canepari, Marco Vogt, Kaspar Zecevic, Dejan
Copyright Year	2008
Abstract	The ability to monitor membrane potential (V m) and calcium (Ca2+) transients at multiple locations on the same neuron can facilitate further progress in our understanding of neuronal function. Here we describe a method to combine V m and Ca2+ imaging using styryl voltage sensitive dyes and Fura type UV-excitable Ca2+ indicators. In all cases V m optical signals are linear with membrane potential changes, but the calibration of optical signals on an absolute scale is presently possible only in some neurons. The interpretation of Ca2+ optical signals depends on the indicator Ca2+ buffering capacity relative to the cell endogenous buffering capacity. In hippocampal CA1 pyramidal neurons, loaded with JPW-3028 and 300 μM Bis-Fura-2, V m optical signals cannot be calibrated and the physiological Ca2+ dynamics are compromised by the presence of the indicator. Nevertheless, at each individual site, relative changes in V m and Ca2+ fluorescence signals under different conditions can provide meaningful new information on local dendritic integration. In cerebellar Purkinje neurons, loaded with JPW-1114 and 1 mM Fura-FF, V m optical signals can be calibrated in terms of mV and Ca2+ optical signals quantitatively reveal the physiological changes in free Ca2+. Using these two examples, the method is explained in detail.
Starting Page	1079
Ending Page	1093
Page Count	15
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	28
Issue Number	8
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2008-05-24
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Voltage-sensitive dyes Calcium-sensitive dyes Imaging Neuronal dendrites Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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