NDLI: Upregulation of Homer1a Promoted Retinal Ganglion Cell Survival After Retinal Ischemia and Reperfusion via Interacting with Erk Pathway

Content Provider	Springer Nature Link
Author	Fei, Fei Li, Juan Rao, Wei Liu, Wenbo Chen, Xiaoyan Su, Ning Wang, Yusheng Fei, Zhou
Copyright Year	2015
Abstract	Retinal ischemia and reperfusion (I/R) is extensively involved in ocular diseases, causing retinal ganglion cell (RGCs) death resulting in visual impairment and blindness. Homer1a is considered as an endogenous neuroprotective protein in traumatic brain injury. However, the roles of Homer1a in RGCs I/R injury have not been elucidated. The present study investigated the changes in expression and effect of Homer1a in RGCs both in vitro and in vivo after I/R injury using Western blot, TUNEL assay, gene interference and overexpression, and gene knockout procedures. The levels of Homer1a and phosphorylated Erk (p-Erk) increased in RGCs and retinas after I/R injury. Upregulation of Homer1a in RGCs after I/R injury decreased the level of p-Erk, and mitigated RGCs apoptosis. Conversely, downregulation of Homer1a increased the level of p-Erk, and augmented RGCs apoptosis. Furthermore, inhibition of the p-ERK reduced RGCs apoptosis, and increased the expression of Homer 1a after I/R injury. Finally, the retinas of Homer1a KO mice treated with I/R injury had significantly less dendrites and RGCs, compared with Homer1a WT mice. These findings demonstrated that Homer1a may contribute to RGCs survival after I/R injury by interacting with Erk pathway.
Starting Page	1039
Ending Page	1048
Page Count	10
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	35
Issue Number	7
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2015-04-30
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Homer1a Erk signaling Retinal ischemia and reperfusion Retinal ganglion cells Neurosciences Cell Biology Neurobiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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