NDLI: Cyclic Nucleotides Induce Long-Term Augmentation of Glutamate-Activated Chloride Current in Molluscan Neurons

Content Provider	Springer Nature Link
Author	Bukava, Julia V. Solntseva, Elena I. Skrebitsky, Vladimir G.
Copyright Year	2005
Abstract	1.Literature data indicate that serotonin induces the long-term potentiation of glutamate (Glu) response in molluscan neurons. The aim of present work was to elucidate whether cyclic nucleotides can cause the same effect. 2.Experiments were carried out on isolated neurons of the edible snail (Helix pomatia) using a two-microelectrode voltage-clamp method. 3.In the majority of the cells examined, the application of Glu elicited a Cl−-current. The reversal potential (Er) of this current lied between −35 and −55 mV in different cells. 4.Picrotoxin, a blocker of Cl−-channels, suppressed this current equally on both sides of Er. Furosemide, an antagonist of both Cl−-channels and the Na+/K+/Cl−-cotransporter, had a dual effect on Glu-response: decrease in conductance, and shift of Er to negative potentials. 5.A short-term (2 min) cell treatment with 8-Br-cAMP or 8-Br-cGMP caused long-term (up to 30 min) change in Glu-response. At a holding potential of −60 mV, which was close to the resting level, an increase in Glu-activated inward current was observed. This potentiation seems to be related to the right shift of Er of Glu-activated Cl−-current rather than to the increase in conductance of Cl−-channels. The blocking effect of picrotoxin rested after 8-Br-cAMP treatment. 6.The change in the Cl−-homeostasis as a possible mechanism for the observed effect of cyclic nucleotides is discussed.
Starting Page	1185
Ending Page	1194
Page Count	10
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	25
Issue Number	8
e-ISSN	15736830
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2005-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	cyclic AMP cyclic GMP potentiation glutamate response chloride current molluscan neurons Neurosciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in