NDLI: Immobilization of lipase onto cellulose ultrafine fiber membrane for oil hydrolysis in high performance bioreactor

Content Provider	Springer Nature Link
Author	Chen, Peng Cheng Huang, Xiao Jun Huang, Fu Ou, Yang Chen, Ming Rui Xu, Zhi Kang
Copyright Year	2011
Abstract	Practical application of biphasic enzyme-immobilized membrane bioreactors (EMBR) requires efficient loading of the enzyme with retention of enzymatic activity. Here, we report a method to fabricate an ultrafine fiber membrane conjugated to lipase with high levels of enzyme loading and activity retention. A cellulose acetate (CA) non-woven ultrafine fiber membrane was prepared with 200 nm nominal fiber diameter by electrospinning, followed by alkaline hydrolysis to obtain regenerated cellulose (RC). The RC ultrafine fiber membrane was oxidized by exposure to NaIO4, simultaneously generating aldehyde groups to couple with pentaethylenehexamine (PEHA) as a spacer for lipase immobilization. A biphasic EMBR was assembled with the PEHA-modified and lipase-immobilized membranes. The effect of operation variables, namely aqueous-phase system, reaction pH, accelerant (sodium taurocholate) content, reaction temperature, and membrane usage on the performance of this bioreactor was investigated with the hydrolysis of olive oil. A bioreactor activity as high as 9.83 × 104 U/m2 was obtained under optimum operational conditions.
Starting Page	1563
Ending Page	1571
Page Count	9
File Format	PDF
ISSN	09690239
Journal	Cellulose
Volume Number	18
Issue Number	6
e-ISSN	1572882X
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellulose Ultrafine fiber membrane Enzyme immobilization Lipase Bioreactor Polymer Sciences Physical Chemistry Organic Chemistry Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Polymers and Plastics

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