NDLI: Conformational changes and sequence analysis in cellulase from Aspergillus niger with cationic surfactant

Content Provider	Springer Nature Link
Author	Rastegari, Ali Asghar Bordbar, Abdol Khalegh Mehnati Najafabadi, Vajihe
Copyright Year	2010
Abstract	The present study evaluates the binding of cetylpyridinium chloride (CPC) with cellulase in various experimental conditions using potentiometric, fluorescence spectroscopy and turbidimetric techniques. The analysis of binding curves revealed the existence of two sets of binding sets for CPC. The binding parameters were estimated and interpreted in terms of structural viewpoints of cellulase. The observation of turbidity suggests that CPC molecules individually nucleate around cellulase/CMC complex to form micelle-like structures. Fluorescence spectroscopy analysis of cellulase/CMC-surfactant system showed that these complexes could be compact to elucidate the mechanism of binding cellulase/CMC complex to CPC. The differential response of the enzyme/CMC to surfactant, indicates that the interaction on the complex surface is strongly ionic and hydrophobic(cooperative) in nature. A sequencing analysis was also conducted on β-1, 4-endoglucanase from A. niger (EglA) and others from family 12 in order to examine the nature of interaction involved in binding process and structure of carbohydrate-protein complexes. The results suggest that the conserved residues are located in a more hydrophobic microenvironment and apolar area energy is more than polar within enzyme structure.
Starting Page	1213
Ending Page	1225
Page Count	13
File Format	PDF
ISSN	09690239
Journal	Cellulose
Volume Number	17
Issue Number	6
e-ISSN	1572882X
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-10-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cetylpyridinium chloride Cellulase Binding isotherm Surfactant Sequence analysis Surfactant selective membrane electrode (SSE) Fluorescence spectroscopy Polymer Sciences Organic Chemistry Physical Chemistry Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Polymers and Plastics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A New PVC Membrane Ion Selective Electrode for Determination of Cationic Surfactant in Mouthwash

Potentiometric study of cetylpyridinium chloride cooperative binding to anionic azo-dyes

Cationic release behaviour of antimicrobial cellulose/silver nanocomposites

Controlled adsorption of cellulase onto pretreated corncob by pH adjustment

Alginic Acid Beads Containing Fluorescent Solvatochromic Dyes Display an Emission Color Response to a Cationic Surfactant.

Effect of cationic surfactant cetyltrimethylammonium bromide on the enzymatic hydrolysis of cellulose

Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose

Simulation analysis of the cellulase Cel7A carbohydrate binding module on the surface of the cellulose Iβ

Reducing end-specific fluorescence labeled celluloses for cellulase mode of action

Conformational changes and sequence analysis in cellulase from Aspergillus niger with cationic surfactant

Similar Documents

A New PVC Membrane Ion Selective Electrode for Determination of Cationic Surfactant in Mouthwash

Potentiometric study of cetylpyridinium chloride cooperative binding to anionic azo-dyes

Cationic release behaviour of antimicrobial cellulose/silver nanocomposites

Controlled adsorption of cellulase onto pretreated corncob by pH adjustment

Alginic Acid Beads Containing Fluorescent Solvatochromic Dyes Display an Emission Color Response to a Cationic Surfactant.

Effect of cationic surfactant cetyltrimethylammonium bromide on the enzymatic hydrolysis of cellulose

Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose

Simulation analysis of the cellulase Cel7A carbohydrate binding module on the surface of the cellulose Iβ

Reducing end-specific fluorescence labeled celluloses for cellulase mode of action

Conformational changes and sequence analysis in cellulase from Aspergillus niger with cationic surfactant