NDLI: Controlled incorporation of deuterium into bacterial cellulose

Content Provider	Springer Nature Link
Author	He, Junhong Pingali, Sai Venkatesh Chundawat, Shishir P. S. Pack, Angela Jones, A. Daniel Langan, Paul Davison, Brian H. Urban, Volker Evans, Barbara O’Neill, Hugh
Copyright Year	2013
Abstract	Isotopic enrichment has been widely used for investigating the structural and dynamic properties of biomacromolecules to provide information that cannot be carried out with molecules composed of natural abundance isotopes. A media formulation for controlled incorporation of deuterium in bacterial cellulose synthesized by Gluconacetobacter xylinus subsp. sucrofermentans is reported. The purified cellulose was characterized using Fourier Transform Infra-Red spectrophotometry and mass spectrometry which revealed that the level of deuterium incorporation in the perdeuterated cellulose was greater than 90 %. Small-angle neutron scattering analysis demonstrated that the overall structure of the cellulose was unaffected by the substitution of deuterium for hydrogen. In addition, by varying the amount of D-glycerol in the media it was possible to vary the scattering length density of the deuterated cellulose. A large disk model was used to fit the curves of bacterial cellulose grown using 0 and 100 % D-Glycerol yielding a lower bound to the disk radii, R min = 1,132 ± 6 and 1,154 ± 3 Å and disk thickness, T = 128 ± 1 and 83 ± 1 Å for the protiated and deuterated forms of the bacterial cellulose, respectively. This agrees well with the scanning electron microscopy analysis which revealed stacked sheets in the cellulose pellicles. Controlled incorporation of deuterium into cellulose will enable new types of experiments using techniques such as neutron scattering to reveal information about the structure and dynamics of cellulose and its interactions with proteins and other (bio) polymers.
Starting Page	927
Ending Page	936
Page Count	10
File Format	PDF
ISSN	09690239
Journal	Cellulose
Volume Number	21
Issue Number	2
e-ISSN	1572882X
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellulose Deuteration Labeling Small-angle neutron scattering Mass spectrometry Bioorganic Chemistry Physical Chemistry Organic Chemistry Polymer Sciences
Content Type	Text
Resource Type	Article
Subject	Polymers and Plastics

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