NDLI: Comparative evaluation of the action of two different endoglucanases. Part II: On a biobleached acid sulphite pulp

Content Provider	Springer Nature Link
Author	Quintana, Elisabet Valls, Cristina Vidal, Teresa Roncero, M. Blanca
Copyright Year	2015
Abstract	A TCF sulphite pulp, bleached at the laboratory scale with a laccase–violuric acid system and complemented with a pressurized hydrogen peroxide stage, was treated with two endoglucanases, one obtained from Paenibacillus barcinonensis (B) and the other one produced from Cerrena unicolor (F) to improve cellulose reactivity. The treated pulps were evaluated in terms of brightness, viscosity, α-cellulose, water retention value, fibre morphology, Fock solubility, NMR and carbohydrate composition of pulps and liquors. Results revealed that both endoglucanases improved cellulose reactivity, albeit in a different way; thus, B caused no scissions in the cellulose chain and no significant reduction in fibre length, whereas F decreased viscosity and shortened fibre length, leading to lower reactivity value. The liquor composition of soluble carbohydrates released by the enzymatic treatments revealed the B had a processive mode of action since short oligosaccharides, cellobiose and glucose, were obtained. F hydrolysed, from high to low concentration, cellobiose, glucose and cellotriose. Importantly, environmentally friendly dissolving pulp with 90 % Fock solubility was obtained, combining two enzymatic treatments: a laccase–mediator system and then a cellulase from P. barcinonensis (B). In order to improve the quality of final dissolving pulp, a pulp purification step was introduced before the B endoglucanase treatment. The cold caustic extraction lead to reduce the amount of hemicelluloses by 42 % with respect to biobleached pulp, but Fock solubility was also reduced. However, complementing the purification step with F treatment, reduced the amount of hemicelluloses but also improved Fock solubility by 17 %, although some presence of cellulose II was detected by NMR.
Starting Page	2081
Ending Page	2093
Page Count	13
File Format	PDF
ISSN	09690239
Journal	Cellulose
Volume Number	22
Issue Number	3
e-ISSN	1572882X
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-04-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellulose Biobleached pulp Dissolving pulp Endoglucanase Oligosaccharides Fock solubility Bioorganic Chemistry Physical Chemistry Organic Chemistry Polymer Sciences
Content Type	Text
Resource Type	Article
Subject	Polymers and Plastics

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