NDLI: Antioxidant activity in alveolar epithelial type 2 cells of rats during the development of bleomycin injury

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Cell Biology and Toxicology : Volume 13

Antioxidant activity in alveolar epithelial type 2 cells of rats during the development of bleomycin injury

Content Provider	Springer Nature Link
Author	Karam, H. Hurbain Kosmath, I. Housset, B.
Copyright Year	1998
Abstract	Bleomycin (BLM) induces lung inflammation and subsequent fibrosis in human and in animal models. Alveolar epithelial type 2 cells (T2 cells) are known to play a crucial role in the repair process after BLM injury. We hypothesized that resistance of T2 cells to BLM-damage was associated with an increase in their antioxidant system activity. We developed an animal model of lung lesions preceding fibrosis, using daily intraperitoneal administration of BLM (1.5 mg/day over 7 and 14 days). We observed a body weight stablization in BLM-treated rats from the third day. After 14 days of BLM treatment, the number of cells recovered by bronchoalveolar lavage was significantly increased (p<0.05), with a dramatic increase (p<0.01) in the percentage of neutrophils associated with a decrease in macrophage percentage (p<0.01). No evidence of fibrosis was seen by microscopic studies at this time. However, T2 cells in 14-day-treated rats were swollen with enlarged lamellar inclusion bodies. Biochemical study of freshly isolated T2 cells displayed a significant decrease of lactate dehydrogenase (LDH) released by these cells when isolated from 14-day-treated rats as compared with 7-day. By contrast, BLM induced an increase in superoxide dismutase (SOD) and glutathione peroxidase activities. Cell content of glutathione was decreased and γ-glutamyl transpeptidase activity was markedly increased. These results show that BLM induces changes in the antioxidant system of T2 cells, particularly in the glutathione system.
Starting Page	13
Ending Page	22
Page Count	10
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	14
Issue Number	1
e-ISSN	15736822
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Pharmacology/Toxicology Veterinary Medicine Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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