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| Content Provider | Springer Nature Link |
|---|---|
| Author | Chadebech, P. Goidin, D. Jacquet, C. Viac, J. Schmitt, D. Staquet, M J. |
| Copyright Year | 2003 |
| Abstract | Defensins have been identified as key elements of innate immunity against microbial infections. In the present study, human β-defensin-2 (hBD-2) mRNA and peptide expression were evaluated by RT-PCR and Western blotting in normal human keratinocytes, in function of their stage of differentiation. In proliferating, non-differentiating keratinocytes generated in serum-free, low-calcium medium, a very low hBD-2 mRNA expression was found. A significantly higher expression was detected in high-calcium cultivated keratinocytes grown either as monolayers or as multilayers under submerged conditions. In an air–liquid interface culture of keratinocytes, allowing epidermis to be reconstructed, hBD-2 mRNA expression level was significantly higher than in the other conditions and displayed inter-individual variability as observed in native epidermis. The peptide was detected only in reconstructed epidermis. These results indicate that hBD-2 gene expression in normal human keratinocytes is dependent upon their stage of differentiation. The level of expression of hBD-1 mRNA was lower and that of hBD-3 was higher than that of hBD-2 in reconstructed epidermis. Exposure of reconstructed epidermis to bacterial lipopolysaccharide (LPS) resulted in an average 4-fold increase in hBD-2 mRNA 18 h after challenge, but not of hBD-1 and hBD-3 gene expression. These results show the selective regulation of hBD-2-encoding gene in an organotypic epidermal model, in response to LPS. They also provide evidence thatin vitro reconstructed epidermis represents a useful model for studying regulation of expression of β-defensins after skin challenge with pathogenic microorganisms in conditions as close as possible to thein vivo situation. |
| Starting Page | 313 |
| Ending Page | 324 |
| Page Count | 12 |
| File Format | |
| ISSN | 07422091 |
| Journal | Cell Biology and Toxicology |
| Volume Number | 19 |
| Issue Number | 5 |
| e-ISSN | 15736822 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2003-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | Subscribed |
| Subject Keyword | Pharmacology/Toxicology Biochemistry Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Health, Toxicology and Mutagenesis Toxicology |
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