NDLI: Cytotoxicity of Nephrotoxic Fungal Toxins to Kidney-derived LLC-PK1 and OK Cell Lines

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Cell Biology and Toxicology : Volume 14, Issue 4, August 1998

Cell Biology and Toxicology : Volume 14, Issue 3, June 1998

Cell Biology and Toxicology : Volume 14, Issue 2, March 1998

Cell Biology and Toxicology : Volume 14, Issue 1, February 1998

Cell Biology and Toxicology : Volume 13

Cytotoxicity of Nephrotoxic Fungal Toxins to Kidney-derived LLC-PK1 and OK Cell Lines

Content Provider	Springer Nature Link
Author	Bondy, G.S. Armstrong, C.L.
Copyright Year	1998
Abstract	The nephrotoxic fungal toxins ochratoxin A (OA), ochratoxin B (OB) and citrinin (CIT) are natural contaminants of foods and feeds. While cytotoxicity assays have proven useful for establishing relative toxicity and structure–function relationships within groups of fungal toxins, a drawback of in vitro bioassays is their susceptibility to variation depending on endpoint, target cell, and dosing strategy. These variables were explored for OA, OB, CIT using two continuous kidney cell lines (LLC-PK1 and OK) and four cytotoxicity assay endpoints. The nephrotoxic antibiotic gentamicin was used as a positive control for cytotoxicity throughout. In general, fungal toxin-induced cytotoxicity was more pronounced in LLC-PK1 cultures using mitochondrial dehydrogenase inhibition (MTT assay) as the endpoint. Altered dosing strategy, but not seeding density, consistently influenced cytotoxicity: CIT was more toxic to cells when added at the time of seeding, whereas OA was more toxic when added 24 h after cultures were seeded. Toxicity rankings for the fungal toxins were consistent with in vivo studies and were, in order of most to least toxic, OA>OB>CIT. The data indicate that LLC-PK1 and OK cells compare favorably to existing models in terms of sensitivity to nephrotoxic fungal toxins, but also that relatively minor changes in assay protocols can affect the cytotoxicity of individual toxins and comparative toxicity within a group of toxins.
Starting Page	323
Ending Page	332
Page Count	10
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	14
Issue Number	5
e-ISSN	15736822
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Pharmacology/Toxicology Veterinary Medicine Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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