NDLI: A mesenchymal stromal cell line resistant to paclitaxel that spontaneously differentiates into osteoblast-like cells

Content Provider	Springer Nature Link
Author	Pessina, Augusto Sisto, Francesca Coccè, Valentina Cavicchini, Loredana Ciusani, Emilio Gribaldo, Laura Bomi, Arianna
Copyright Year	2010
Abstract	The mesenchymal stromal cell line SR-4987 has been established in our laboratory from the bone marrow of BDF/1 mice. Recent information on mesenchymal stem cells biology and the need to deal with well-characterized cell lines suggest to critically consider the existent data on this cell line by updating them with new investigations on growth parameters, in vitro plasticity, and drug sensitivity to anti-cancer, anti-inflammatory, and a histone deacetylase inhibitor. SR-4987 cells show a population doubling time of 24.5 ± 5.4 h, a plating efficiency of 2.87 ± 1.19%, and under stimulation maintain only in part their multipotency by differentiating towards chondro-osteogenic lineages but not into adipogenic. Surprisingly, these mesenchymal stromal cells differentiate spontaneously into osteoblast-like cells and this is significantly stimulated by valproic acid. SR-4987 cells show a dramatic resistance to paclitaxel (PTX) with a resistance index of 39.6 times (evaluated versus MOLT-4 leukemia) and of 68.2 (versus HT-29 colorectal carcinoma). SR-4987 resistance is reversed by verapamil and correlates with high expression of P-glycoprotein that is down-modulated by PTX. Taken together, our results indicated that SR-4987 line is a very interesting cell model useful to investigate both drug sensitivity resistance and physiopathological aspects related to mesenchymal cell function.
Starting Page	169
Ending Page	180
Page Count	12
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	27
Issue Number	3
e-ISSN	15736822
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-12-29
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Drug sensitivity Mesenchymal stem cells Osteogenesis Paclitaxel resistance SR-4987 Biochemistry Cell Biology Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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