NDLI: In vitro effect of malathion and diazinon on oocytes fertilization and embryo development in porcine

Content Provider	Springer Nature Link
Author	Ducolomb, Yvonne Casas, Eduardo Valdez, Alejandra González, Guadalupe Altamira Loza, Mario Betancourt, Miguel
Copyright Year	2009
Abstract	Diazinon and malathion are commonly used organophosphate insecticides in agriculture, industry, and in veterinary medicine as an ectoparasiticide. The importance to carry out in vitro reproductive toxicology assays lies on the need of knowing the alterations these insecticides may cause at cellular level, since they are endocrine disruptors that interfere with reproductive functions. The aim of this study was to evaluate in vitro oocyte viability, fertilization, and embryo development with different concentrations of diazinon and malathion. For in vitro fertilization (IVF), porcine oocytes and sperm were co-incubated for 7 h with increasing concentrations (50, 100, and 500 μM) of diazinon and malathion. For embryo development, fertilized oocytes were cultured in medium containing the same insecticide concentrations during 96 h for embryo development and 144 h for morulae formation. Diazinon did not affect oocyte viability and embryo divisions but decreased IVF (fertilization inhibition50 = 502 μM) and morulae formation (morulae inhibition50 = 344 μM). Malathion affected all the studied parameters: lethal concentration50 = 1 mM, fertilization inhibition50 = 443 μM, development inhibition50 = 375 μM, and morulae inhibition50 = 216 μM. The results of this study indicate that diazinon and malathion used in commercial formulation could be toxic, producing impairment in in vitro fertilization and embryo development. This is an approach for further investigations to find out cell damage mechanisms produced by these widely used insecticides.
Starting Page	623
Ending Page	633
Page Count	11
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	25
Issue Number	6
e-ISSN	15736822
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-01-17
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Diazinon Embryo development In vitro fertilization Malathion Oocyte Porcine Biochemistry Pharmacology/Toxicology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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