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Establishment of a rat Sertoli cell line that displays the morphological and some of the functional characteristics of the native cell

Author Index to Volume 13

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Cell Biology and Toxicology : Volume 13, Issue 4-5, July 1997

Cell Biology and Toxicology : Volume 13, Issue 3, March 1997

Cell Biology and Toxicology : Volume 13, Issue 2, February 1997

Cell Biology and Toxicology : Volume 13, Issue 1, January 1997

Establishment of a rat Sertoli cell line that displays the morphological and some of the functional characteristics of the native cell

Content Provider	Springer Nature Link
Author	Pognan, F. Masson, M. T. Lagelle, F. Charuel, C.
Copyright Year	1997
Abstract	Primary rat Sertoli cells are widely used as a model for mechanistic and toxicological studies, since they are often the target of toxicants in vivo. However, their isolation from testicular homogenates is tedious and requires the regular use of numerous immature animals. It is therefore of great interest to have available established cell lines that are usable in vitro for unlimited periods and closely similar to native cells. To this end, we have established a line of Wistar rat Sertoli cells (SerW3) by immortalization of fresh primary cells with the T antigens of the Simian virus (SV40). When plated on Matrigel, this cell line presents many of the functional characteristics of Sertoli cells in vivo. In addition, they are sensitive to cisplatin and secrete transferrin, although they do not show a clear response to follicle-stimulating hormone. They also present many morphological similarities, including the presence of tight junctions which mimic the natural epithelial barrier. Like Sertoli cells in vivo, they show extensive phagocytic activity. Finally, they display all the characteristics of immortalized, but not transformed, cells, i.e., topo-inhibition and apoptosis at confluence or under serum deprivation.
Starting Page	453
Ending Page	463
Page Count	11
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	13
Issue Number	6
e-ISSN	15736822
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Pharmacology/Toxicology Veterinary Medicine Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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Establishment of a rat Sertoli cell line that displays the morphological and some of the functional characteristics of the native cell

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