NDLI: Enantiopure Synthesis of (R)-Mandelonitrile Using Hydroxynitrile Lyase of Wild Apricot (Prunus armeniaca L.) [ParsHNL] in Aqueous/Organic Biphasic System

Content Provider	Springer Nature Link
Author	Asif, Mohammad Bhalla, Tek Chand
Copyright Year	2017
Abstract	Hydroxynitrile lyases (HNLs) are increasingly finding application in the synthesis of enantiomerically pure cyanohydrins which are important intermediates in the production of pharmaceuticals and agrochemicals. Synthesis of enantiopure mandelonitrile was carried out using HNL of wild apricot (Prunus armeniaca L.) [ParsHNL] in aqueous/organic biphasic system. The optimum pH and temperature of the reaction were 4.0 and 15 °C respectively, which are important parameters to suppress the non-enzymatic catalysis. ParsHNL catalyses synthesis of (R)-mandelonitrile in methyl-tbutyl ether (MTBE)/citrate buffer biphasic system with >99% ee. Synthesis of mandelonitrile was carried out in batch reaction at 40 ml scale and finally 2.7 mmoles of (R)-mandelonitrile was recovered which corresponded to 90% molar conversion in 46 h reaction. In fed batch reaction 6.37 mmoles of (R)-mandelonitrile could be produced which corresponds to 91% molar conversion in 46 h. In both reactions, enzyme produces (R)-mandelonitrile with > 99% ee which showed enhanced selectivity as compared to aqueous reaction (96% ee) by ParsHNL. The results showed potential of ParsHNL to synthesize (R)-mandelonitrile in both, batch reaction and fed-batch reaction and can be effectively used in the synthesis of (R)-mandelonitrile.
Starting Page	1592
Ending Page	1597
Page Count	6
File Format	PDF
ISSN	1011372X
Journal	Catalysis Letters
Volume Number	147
Issue Number	6
e-ISSN	1572879X
Language	English
Publisher	Springer US
Publisher Date	2017-03-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Prunus armeniaca L. Wild apricot (R)-mandelonitrile Biphasic reaction Hydroxynitrile lyase ParsHNL Cyanohydrin Batch-fed batch reaction Catalysis Industrial Chemistry/Chemical Engineering Organometallic Chemistry Physical Chemistry
Content Type	Text
Resource Type	Article
Subject	Chemistry Catalysis

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