NDLI: Assessment of cryopreserved donor skin viability: the experience of the regional tissue bank of Siena

Content Provider	Springer Nature Link
Author	Pianigiani, E. Tognetti, L. Ierardi, F. Mariotti, G. Rubegni, P. Cevenini, G. Perotti, R. Fimiani, M.
Copyright Year	2016
Abstract	Skin allografts from cadaver donors are an important resource for treating extensive burns, slow-healing wounds and chronic ulcers. A high level of cell viability of cryopreserved allografts is often required, especially in burn surgery, in Italy. Thus, we aimed to determine which conditions enable procurement of highly viable skin in our Regional Skin Bank of Siena. For this purpose, we assessed cell viability of cryopreserved skin allografts procured between 2011 and 2013 from 127 consecutive skin donors, before and after freezing (at day 15, 180, and 365). For each skin donor, we collected data concerning clinical history (age, sex, smoking, phototype, dyslipidemia, diabetes, cause of death), donation process (multi-tissue or multi-organ) and timing of skin procurement (assessment of intervals such as death-harvesting, harvesting-banking, death-banking). All these variables were analysed in the whole case study (127 donors) and in different groups (e.g. multi-organ donors, non refrigerated multi-tissue donors, refrigerated multi-tissue donors) for correlations with cell viability. Our results indicated that cryopreserved skin allografts with higher cell viability were obtained from female, non smoker, heartbeating donors died of cerebral haemorrhage, and were harvested within 2 h of aortic clamping and banked within 12 h of harvesting (13–14 h from clamping). Age, cause of death and dyslipidaemia or diabetes did not appear to influence cell viability. To maintain acceptable cell viability, our skin bank needs to reduce the time interval between harvesting and banking, especially for refrigerated donors.
Starting Page	241
Ending Page	253
Page Count	13
File Format	PDF
ISSN	13899333
Journal	Cell and Tissue Banking
Volume Number	17
Issue Number	2
e-ISSN	15736814
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-03-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cryopreserved skin allografts Cadaver donor Skin viability Skin tissue bank Cell Biology Biomedicine general Transplant Surgery
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biomaterials Transplantation Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Assessment of cryopreserved donor skin viability: the experience of the regional tissue bank of Siena.

Viability of cryopreserved human skin allografts: effects of transport media and cryoprotectant

Bacterial Contamination of Tissue Allografts – Experiences of the Donor Tissue Bank of Victoria

The role of quality control in a skin bank: tissue viability determination.

Assessment of a tumor bank: a thirty years experience of the University of Siena (Italy)

Evaluation of a microbiological screening and acceptance procedure for cryopreserved skin allografts based on 14 day cultures

Skin donors and human skin allografts: evaluation of an 11-year practice and discard in a referral tissue bank

Assessment of organ culture for the conservation of human skin allografts

Severe adult burn survivors. What information about skin allografts?

Assessment of cryopreserved donor skin viability: the experience of the regional tissue bank of Siena

Similar Documents

Assessment of cryopreserved donor skin viability: the experience of the regional tissue bank of Siena.

Viability of cryopreserved human skin allografts: effects of transport media and cryoprotectant

Bacterial Contamination of Tissue Allografts – Experiences of the Donor Tissue Bank of Victoria

The role of quality control in a skin bank: tissue viability determination.

Assessment of a tumor bank: a thirty years experience of the University of Siena (Italy)

Evaluation of a microbiological screening and acceptance procedure for cryopreserved skin allografts based on 14 day cultures

Skin donors and human skin allografts: evaluation of an 11-year practice and discard in a referral tissue bank

Assessment of organ culture for the conservation of human skin allografts

Severe adult burn survivors. What information about skin allografts?

Assessment of cryopreserved donor skin viability: the experience of the regional tissue bank of Siena