NDLI: Isolation of human dermis derived mesenchymal stem cells using explants culture method: expansion and phenotypical characterization

Content Provider	Springer Nature Link
Author	Park, Jeong Ran Kim, Eunjeong Yang, Jungwon Lee, Hanbyeol Hong, Seok Ho Woo, Heung Myong Park, Sung Min Na, Sunghun Yang, Se Ran
Copyright Year	2014
Abstract	Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigated to determine the potential of stem cell within human skin dermis. We isolated cells from human dermis, termed here as human dermis-derived mesenchymal stem cells (hDMSCs) which is able to be isolated by using explants culture method. Our method has an advantage over the enzymatic method as it is easier, less expensive and less cell damage. hDMSCs were maintained in basal culture media and proliferation potential was measured. hDMSCs were highly proliferative and successfully expanded with no additional growth factor. In addition, hDMSCs revealed normal karyotype and expressed high levels of CD90, CD73 and CD105 while did not express the surface markers for CD34, CD45 and HLA-DR. Also, we confirmed that hDMSCs possess the capacity to differentiate into multiple lineage including adipocyte, osteocyte, chondrocyte and precursor of hepatocyte lineage. Considering these results, we suggest that hDMSCs might be a valuable source of stem cells and could potentially be a useful source of clinical application.
Starting Page	209
Ending Page	218
Page Count	10
File Format	PDF
ISSN	13899333
Journal	Cell and Tissue Banking
Volume Number	16
Issue Number	2
e-ISSN	15736814
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-08-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human dermis Mesenchymal stem cells Explants culture method Differentiation potential Cell Biology Biomedicine general Transplant Surgery
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biomaterials Transplantation Biomedical Engineering

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