NDLI: Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

Content Provider	Springer Nature Link
Author	Maezawa, Yasuyo Oka, Kazuri Matsubara, Mie Masuhara, Hiroshi Hosokawa, Yoichiroh
Copyright Year	2010
Abstract	When nerve growth factor (NGF) is interacted with PC12 cells derived from rat pheochromocytoma, they are partially differentiated into neuron-like cells with neurites. In this work, PC12 cells differentiated by NGF were selectively isolated using a localized impulsive force in a μm-scale area, which was generated by focusing an infrared femtosecond laser into a cell culture medium. In order to evaluate the ability of the isolation method, differentiated and undifferentiated cells were isolated and their morphological changes after the isolation were compared. In both cases, their neurites were once contracted and some of them gradually regenerated day by day. When differentiated cells were isolated, the percentage of differentiated cells with regenerated neurites, 6 h after the isolation, was about 3.3 times higher than that when undifferentiated ones were isolated. This result was compared with a control trypsin experiment. In the comparison, it was indicated that the same degree of cell function was maintained when the present isolation method was used.
Starting Page	117
Ending Page	122
Page Count	6
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	13
Issue Number	1
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2010-10-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Femtosecond laser Laser manufacturing Impulsive force Cell Isolation Cell differentiation Engineering Fluid Dynamics Nanotechnology Biophysics and Biological Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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