NDLI: Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays

Content Provider	Springer Nature Link
Author	Zhu, Ying Warrick, Jay W. Haubert, Kathryn Beebe, David J. Yin, John
Copyright Year	2008
Abstract	The plaque assay has long served as the “gold standard” to measure virus infectivity and test antiviral drugs, but the assay is labor-intensive, lacks sensitivity, uses excessive reagents, and is hard to automate. Recent modification of the assay to exploit flow-enhanced virus spread with quantitative imaging has increased its sensitivity. Here we performed flow-enhanced infection assays in microscale channels, employing passive fluid pumping to inoculate cell monolayers with virus and drive infection spread. Our test of an antiviral drug (5-fluorouracil) against vesicular stomatitis virus infections of BHK cell monolayers yielded a two-fold improvement in sensitivity, relative to the standard assay based on plaque counting. The reduction in scale, simplified fluid handling, image-based quantification, and higher assay sensitivity will enable infection measurements for high-throughput drug screening, sero-conversion testing, and patient-specific diagnosis of viral infections.
Starting Page	565
Ending Page	570
Page Count	6
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	11
Issue Number	3
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2009-01-14
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidic device Infection assay Antiviral drug Passive pumping Quantitative imaging Engineering Fluid Dynamics Nanotechnology Biophysics/Biomedical Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays

Cell loss in integrated microfluidic device

A microfluidic chip for permeability assays of endothelial monolayer

Integrated microfluidic devices for combinatorial cell-based assays

Drug effects analysis on cells using a high throughput microfluidic chip

A compact microfluidic gradient generator using passive pumping

Microfluidic self-assembly of tumor spheroids for anticancer drug discovery

Sensitive on-chip quantitative real-time PCR performed on an adaptable and robust platform

Retina-on-a-chip: a microfluidic platform for point access signaling studies

Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays

Similar Documents

Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays

Cell loss in integrated microfluidic device

A microfluidic chip for permeability assays of endothelial monolayer

Integrated microfluidic devices for combinatorial cell-based assays

Drug effects analysis on cells using a high throughput microfluidic chip

A compact microfluidic gradient generator using passive pumping

Microfluidic self-assembly of tumor spheroids for anticancer drug discovery

Sensitive on-chip quantitative real-time PCR performed on an adaptable and robust platform

Retina-on-a-chip: a microfluidic platform for point access signaling studies

Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays