NDLI: Production of arrays of cardiac and skeletal muscle myofibers by micropatterning techniques on a soft substrate

Content Provider	Springer Nature Link
Author	Cimetta, Elisa Pizzato, Sara Bollini, Sveva Serena, Elena Coppi, Paolo Elvassore, Nicola
Copyright Year	2008
Abstract	Micropatterning and microfabrication techniques have been widely used to pattern cells on surfaces and to have a deeper insight into many processes in cell biology such as cell adhesion and interactions with the surrounding environment. The aim of this study was the development of an easy and versatile technique for the in vitro production of arrays of functional cardiac and skeletal muscle myofibers using micropatterning techniques on soft substrates. Cardiomyocytes were used for the production of oriented cardiac myofibers whereas mouse muscle satellite cells for that of differentiated parallel myotubes. We performed micro-contact printing of extracellular matrix proteins on soft polyacrylamide-based hydrogels photopolymerized onto functionalized glass slides. Our methods proved to be simple, repeatable and effective in obtaining an extremely selective adhesion of both cardiomyocytes and satellite cells onto patterned soft hydrogel surfaces. Cardiomyocytes resulted in aligned cardiac myofibers able to exhibit a synchronous contractile activity after 2 days of culture. We demonstrated for the first time that murine satellite cells, cultured on a soft hydrogel substrate, fuse and form aligned myotubes after 7 days of culture. Immunofluorescence analyses confirmed correct expression of cell phenotype, differentiation markers and sarcomeric organization. These results were obtained in myotubes derived from satellite cells from both wild type and MDX mice which are research models for the study of muscle dystrophy. These arrays of both cardiac and skeletal muscle myofibers could be used as in vitro models for pharmacological screening tests or biological studies at the single fiber level.
Starting Page	389
Ending Page	400
Page Count	12
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	11
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2008-11-06
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Micro-patterning Soft substrate Hydrogel Cardiomyocytes Satellite cells Cell array Engineering Fluid Dynamics Nanotechnology Biophysics/Biomedical Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hydrogel cell patterning incorporating photocaged RGDS peptides

Print-to-print: a facile multi-object micro-patterning technique

Micro-spot, UV and wetting patterning pathways for applications of biofunctional aminosilane-titanate coatings

An integrated device for patterning cells and selectively detaching

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays

A contactless electrical stimulator: application to fabricate functional skeletal muscle tissue

Microfluidic patterning for fabrication of contractile cardiac organoids

Microcontact printing and microspotting as methods for direct protein patterning on plasma deposited polyethylene oxide: application to stem cell patterning

Production of arrays of cardiac and skeletal muscle myofibers by micropatterning techniques on a soft substrate

Similar Documents

Hydrogel cell patterning incorporating photocaged RGDS peptides

Print-to-print: a facile multi-object micro-patterning technique

Micro-spot, UV and wetting patterning pathways for applications of biofunctional aminosilane-titanate coatings

An integrated device for patterning cells and selectively detaching

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays

A contactless electrical stimulator: application to fabricate functional skeletal muscle tissue

Microfluidic patterning for fabrication of contractile cardiac organoids

Microcontact printing and microspotting as methods for direct protein patterning on plasma deposited polyethylene oxide: application to stem cell patterning

Production of arrays of cardiac and skeletal muscle myofibers by micropatterning techniques on a soft substrate