NDLI: Highly efficient capture and harvest of circulating tumor cells on a microfluidic chip integrated with herringbone and micropost arrays

Content Provider	Springer Nature Link
Author	Xue, Peng Wu, Yafeng Guo, Jinhong Kang, Yuejun
Copyright Year	2015
Abstract	Circulating tumor cells (CTCs), which are derived from primary tumor site and transported to distant organs, are considered as the major cause of metastasis. So far, various techniques have been applied for CTC isolation and enumeration. However, there exists great demand to improve the sensitivity of CTC capture, and it remains challenging to elute the cells efficiently from device for further biomolecular and cellular analyses. In this study, we fabricate a dual functional chip integrated with herringbone structure and micropost array to achieve CTC capture and elution through EpCAM-based immunoreaction. Hep3B tumor cell line is selected as the model of CTCs for processing using this device. The results demonstrate that the capture limit of Hep3B cells can reach up to 10 cells (per mL of sample volume) with capture efficiency of 80 % on average. Moreover, the elution rate of the captured Hep3B cells can reach up to 69.4 % on average for cell number ranging from 1 to 100. These results demonstrate that this device exhibits dual functions with considerably high capture rate and elution rate, indicating its promising capability for cancer diagnosis and therapeutics.
Starting Page	1
Ending Page	8
Page Count	8
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	17
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2015-03-08
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Circulating tumor cells Herringbone Micropost Cell capture and harvest Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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