NDLI: Single step neutravidin patterning: a lithographic approach for patterning proteins

Content Provider	Springer Nature Link
Author	Verma, Sankalp Belay, Mezigebu Verma, Vivek
Copyright Year	2016
Abstract	Protein patterning on surfaces is studied extensively for its potential use in proteomic, nanostructures, drug delivery and sensing. Patterning of proteins at micro and nano scales is especially important not only to understand the function of patterned protein but also to study its interaction with subsequent layers of bio-molecules/cells. Micro scale protein patterning is especially difficult due to the fragile nature of proteins. The already available methods either involve complex chemistries or are specific to a few proteins. Thus, in this regard, a versatile approach to pattern proteins using neutravidin is developed. With this approach of lithography and subsequent lift-off of the photoresist, any biotinylated moiety can be patterned at micron scale resolution. Functionality of patterned neutravidin is confirmed by showing binding of biotinylated polystyrene beads and biotinylated antibodies. In addition, stronger physisorption of neutravidin on bare glass surface, as a result of acetone lift-off, helps sustain the protein layers onto the glass surface without the need of chemical immobilization.
Starting Page	1
Ending Page	6
Page Count	6
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	18
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2016-02-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	NeutrAvidin Photolithography Protein patterning Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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