NDLI: Development of a Novel Hand-Held Immunoassay for the Detection of Enterohemorrhagic Escherichia coli O157:H7

Content Provider	Springer Nature Link
Author	Lin, Frank Y.H. Sherman, Philip M. Li, Dongqing
Copyright Year	2004
Abstract	Escherichia coli O157 : H7 is an important human pathogen responsible for numerous outbreaks of hemorrhagic colitis and hemolytic uremic syndrome world-wide. A portable detection device is needed for field testing and point-of-care testing. Poly(dimethylsiloxane) (PDMS) is a solid substrate well suited for adsorption of macromolecules. The purpose of this study was to develop a hand-held enzyme-linked immunosorbent assay (ELISA) for the detection of E. coli O157 : H7 antigens. The prototype consisted of three modules: one for loading reagents, a second for immunosensor detection, and a third for discharge of wastes. Reagent delivery was achieved by using 1-ml syringes embedded within the loading module. The detection module was based on a PDMS layer on which varying concentrations of E. coli O157 : H7 antigens, and negative controls (Lactobacillus rhamnosus R011 and phosphate-buffered saline) were passively adsorbed. Commercially available goat anti-E. coli O157 : H7 antibody was used as a primary antibody, a donkey anti-goat IgG conjugated with horseradish peroxidase was used as a secondary antibody, and a precipitating substrate was employed for colorimetric detection. Results obtained with the prototype were compared to those obtained using a conventional nitrocellulose membrane-based immunoassay. Using the prototype, E. coli O157 : H7 antigens, in quantities from 4 to 400 ng, were accurately detected. This detection limit was comparable to that observed using conventional dot-blot assays. The PDMS layer could be re-used without loss of sensitivity. Colorimetric detection could be visualized readily without the need for sophisticated equipment. Furthermore, this device can be adapted to perform diagnostics for other microbial pathogens currently detected using immunoassay methodology.
Starting Page	125
Ending Page	130
Page Count	6
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	6
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Characterization and Evaluation Materials Pharmacy Biophysics/Biomedical Physics Electronic and Computer Engineering Circuits and Systems Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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