NDLI: Dynamic monitoring of single cell lysis in an impedance-based microfluidic device

Content Provider	Springer Nature Link
Author	Zhou, Ying Basu, Srinjan Laue, Ernest D. Seshia, Ashwin A.
Copyright Year	2016
Abstract	A microfluidic device that is capable of trapping and sensing dynamic variations in the electrical properties of individual cells is demonstrated. The device is applied to the real-time recording of impedance measurements of mouse embryonic stem cells (mESCs) during the process of membrane lysis, with the resulting changes in the electrical properties of cells during this process being quantitatively tracked over time. It is observed that the impedance magnitude decreases dramatically after cell membrane lysis. A significant shift in the phase spectrum is also observed during the time course of this process. By fitting experimental data to physical models, the electrical parameters of cells can be extracted and parameter variations quantified during the process. In the cell lysis experiments, the equivalent conductivity of the cell membrane is found to increase significantly due to pore formation in the membrane during lysis. An increase in the specific capacitance of the membrane is also observed. On the other hand, the conductivity of the cytoplasm is observed to decrease, which may be explained the fact that excess water enters the cell through the gradual permeabilization of the membrane during lysis. Cells can be trapped in the device for periods up to several days, and their electrical response can be monitored by real-time impedance measurements in a label-free and non-invasive manner. Furthermore, due to the highly efficient single cell trapping capacity of the device, a number of cells can be trapped and held in separate wells for concurrent parallel experiments, allowing for the possibility of stepped parametric experiments and studying cell heterogeneity by combining measurements across the array.
Starting Page	1
Ending Page	10
Page Count	10
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	18
Issue Number	4
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2016-06-14
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Single cell trapping Impedance spectroscopy Stem cells Real-time measurements Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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