NDLI: An automatic microturbidostat for bacterial culture at constant density

Content Provider	Springer Nature Link
Author	Luo, Xianjia Shen, Kangyang Luo, Chunxiong Ji, Hang Ouyang, Qi Chen, Yong
Copyright Year	2010
Abstract	We have developed a microturbidostat for long time bacterial culture at constant density controlled by optical detection and integrated pneumatic valves. The device was fabricated by multilayer soft lithography and in-situ formation of an agarose filter. The culture chamber of bacteria was connected in one side to a single bacterial input-output channel and in another side to a nutrient channel in which the agarose filter was formed to ensure the diffusion of nutrients and metabolites without bacterial loss. The bacterial number in the culture chamber was determined by measuring the fluorescence intensity of GFP proteins of the bacteria and the redundant bacteria could be exported automatically through the input-output channel with integrated micro-valves. In order to optimize the operation performance, we investigated the bacterial exportation efficiency with different input-output channel widths. As expected, the bacterial sorting coefficient was proportional to the input-output channel width. The results also showed that with a 20 µm channel-width, a long time culture was possible with a constant bacterial number in the chamber in the range from 400 to 700.
Starting Page	499
Ending Page	503
Page Count	5
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	12
Issue Number	3
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2010-02-23
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Microturbidostat Bacterial growth Engineering Fluid Dynamics Nanotechnology Biophysics and Biological Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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