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| Content Provider | Springer Nature Link |
|---|---|
| Author | Yang, Wei Li, Zhaojun Zhang, Weilin Luo, Chunxiong Ouyang, Qi Yang, Gen Wang, Yugang |
| Copyright Year | 2013 |
| Abstract | In this paper, we propose a novel cell self-loading and patterning device for quantitatively study density effect on cell behaviors. Using this device, it is easy to gather different cell density colonies in different sizes of micro-chambers using one homogeneous cell solution. As a demonstration, we show that the cell number of self-patterning MCF-7 colony is in proportion to the size of liquid-absorbing cavity in the device, from single cell to tens of cells. This device can easily be used to compare the cancer cells’ proliferation in different micro-environments, such as the same number of cells in micro-cavities with different sizes, or different numbers of cells in micro-cavities with the same size, or with different FBS concentrations. Our studies imply a plausible positive correlation between the local concentration of autocrine factors and tumor cell proliferation, which is also quantitative analyzed by a simple model. |
| Starting Page | 683 |
| Ending Page | 689 |
| Page Count | 7 |
| File Format | |
| ISSN | 13872176 |
| Journal | Biomedical Microdevices |
| Volume Number | 15 |
| Issue Number | 4 |
| e-ISSN | 15728781 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2013-06-20 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cell self-loading Density control Cancer autocrine effect Tumor proliferation Biomedical Engineering Biological and Medical Physics, Biophysics Nanotechnology Engineering Fluid Dynamics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering |
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