NDLI: Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses

Content Provider	Springer Nature Link
Author	Ziv, Roee Steinhardt, Yair Pelled, Gadi Gazit, Dan Rubinsky, Boris
Copyright Year	2008
Abstract	Micro-electroporation is an electroporation technology in which the electrical field that induces cell membrane poration is focused onto a single cell contained in a micro-electromechanical structure. Micro-electroporation has many unique attributes including that it facilitates real time control over the process of electroporation at the single cell level. Flow-through micro-electroporation expands on this principle and was developed to facilitate electroporation of a large numbers of cells with control over the electroporation of every single cell. However, our studies show that when electroporation employs conventional direct current (DC) electrical pulses the micro-electroporation system fails, because of electrolysis induced gas bubble formation. We report in this study that when certain alternating currents (AC) electrical pulses are used for micro-electroporation it becomes possible to avoid electrolytic gas bubble formation in a micro-electroporation flow-through system. The effect of AC micro-electroporation on electrolysis was found to depend on the AC frequency used. This concept was tested with mesenchymal stem cells and preliminary results show successful electroporation using this system.
Starting Page	95
Ending Page	101
Page Count	7
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	11
Issue Number	1
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2008-09-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Micro-electroporation Mesenchymal stem cells Micro-channel Cell membrane Transmembrane potential Electrolysis Engineering Fluid Dynamics Nanotechnology Biophysics/Biomedical Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A primary current distribution model of a novel micro-electroporation channel configuration

Model of pore formation in a single cell in a flow-through channel with micro-electrodes

Electrical and thermal characterization of nanochannels between a cell and a silicon based micro-pore

Delivery of molecules into cells using localized single cell electroporation on ITO micro-electrode based transparent chip

Fabrication of electro-microfluidic channel for single cell electroporation

Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells

Electrical impedance tomographic imaging of a single cell electroporation

Electrophoresis-assisted single-cell electroporation for efficient intracellular delivery

A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate

Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses

Similar Documents

A primary current distribution model of a novel micro-electroporation channel configuration

Model of pore formation in a single cell in a flow-through channel with micro-electrodes

Electrical and thermal characterization of nanochannels between a cell and a silicon based micro-pore

Delivery of molecules into cells using localized single cell electroporation on ITO micro-electrode based transparent chip

Fabrication of electro-microfluidic channel for single cell electroporation

Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells

Electrical impedance tomographic imaging of a single cell electroporation

Electrophoresis-assisted single-cell electroporation for efficient intracellular delivery

A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate

Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses