NDLI: A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot

Content Provider	Springer Nature Link
Author	Hou, Jie Shi, Qian Cao, Meirong Pan, Pengwei Ge, Guangbo Fan, Xuran Bai, Gang Xin, Yi
Copyright Year	2014
Abstract	Glycyrrhetic acid (GA), a pentacyclic triterpenoid derivative obtained from hydrolysis of glycyrrhizic acid, was found to have synergistic anti-asthmatic effects with the β2-adrenergic receptor (β2AR) agonist via the β2AR-mediated pathway. This study visualized the location of GA on a human cell expressing β2AR via chemical biological approaches. A CdTe/ZnS quantum dot modified with an alkynyl group (QD-AL) was first synthesized, and an azide-terminal GA (ATGA) was also prepared. The QD-AL was used for fluorescence visualization of the distribution of GA on human embryonic kidney 293 cells expressing fusion β2AR (HEK293-β2AR) through the “click reaction” between QD-AL and ATGA. The average size of the QD-AL nanoparticle was about 10 nm, and its fluorescent emission wavelength was 620 nm. The location of GA on the HEK293-β2AR cell membrane can be visualized by the click reaction (between QD-AL and ATGA). The ability of QD-AL targeting to ATGA on the cell membrane of a HEK293-β2AR cell was further investigated using both confocal laser-scanning microscopy and a cellular uptake-inhibition assay. The results reveal that QD-AL can recognize ATGA on the cell membrane through the click reaction, which provides a new approach for visualizing the location of GA on the cell in an indirect way, and it can be applied to explore the synergistic anti-asthmatic mechanism of GA with β2AR agonist through the β2AR mediated pathway.
Starting Page	25
Ending Page	30
Page Count	6
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	79
Issue Number	1
e-ISSN	16083040
Language	English
Publisher	Springer US
Publisher Date	2014-01-30
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	fluorescent visualization glycyrrhetic acid quantum dot click reaction Biochemistry Bioorganic Chemistry Microbiology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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