NDLI: Solid-phase assays for study of carbohydrate specificity of galectins

Content Provider	Springer Nature Link
Author	Rapoport, E. M. Pochechueva, T. V. Kurmyshkina, O. V. Pazynina, G. V. Severov, V. V. Gordeeva, E. A. Belyanchikov, I. M. Andre, S. Gabius, H. J. Bovin, N. V.
Copyright Year	2010
Abstract	We have recently shown that the carbohydrate-binding pattern of galectins in cells differs from that determined in artificial (non-cellular) test-systems. To understand the observed discrepancy, we compared several test-systems differing in the mode of galectin presentation on solid phase. The most representative system was an assay where the binding of galectin (human galectins-1 and -3 were studied) to asialofetuin immobilized on solid phase was inhibited by polyacrylamide glycoconjugates, Glyc-PAA. This approach permits us to range quantitatively glycans (Glyc) by their affinity to galectin, i.e. to study both high and low affinity ligands. Our attempts to imitate the cell system by solid-phase assay were not successful. In the cell system galectin binds glycoconjugates by one carbohydrate-recognizing domain (CRD), and after that the binding to the remaining non-bound CRD is studied by means of fluorescein-labeled Glyc-PAA. In an “imitation” variant when galectins are loaded on adsorbed asialofetuin or Glyc-PAA followed by revealing of binding by the second Glyc-PAA, the interaction was not observed or glycans were ordered poorly, unlike in the inhibitory assay. When galectins were adsorbed on corresponding antibodies (when all CRDs were free for recognition by carbohydrate), a good concentration dependence was observed and patterns of specificities were similar (though not identical) for the two methods; notably, this system does not reflect the situation in the cell. Besides the above-mentioned, other variants of solid-phase analysis of galectin specificity were tested. The results elucidate the mechanism and consequence of galectin CRD cis-masking on cell surface.
Starting Page	310
Ending Page	319
Page Count	10
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	75
Issue Number	3
e-ISSN	16083040
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-03-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	galectins glycoconjugates masking solid-phase assay oligosaccharides carbohydrate specificity Biomedicine general Microbiology Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mammalian galectins: Structure, carbohydrate specificity, and functions

Specificity of human galectins on cell surfaces

Carbohydrate specificity of chicken and human tandem-repeat-type galectins-8 in composition of cells

Probing sialic acid binding Ig-like lectins (siglecs) with sulfated oligosaccharides

Galectins promote the interaction of influenza virus with its target cell

Plant oligosaccharides — outsiders among elicitors?

Britton Chance

Microbial carbohydrates

An easy colorimetric assay for glycosyltransferases

Solid-phase assays for study of carbohydrate specificity of galectins

Similar Documents

Mammalian galectins: Structure, carbohydrate specificity, and functions

Specificity of human galectins on cell surfaces

Carbohydrate specificity of chicken and human tandem-repeat-type galectins-8 in composition of cells

Probing sialic acid binding Ig-like lectins (siglecs) with sulfated oligosaccharides

Galectins promote the interaction of influenza virus with its target cell

Plant oligosaccharides — outsiders among elicitors?

Britton Chance

Microbial carbohydrates

An easy colorimetric assay for glycosyltransferases

Solid-phase assays for study of carbohydrate specificity of galectins