NDLI: Hepatitis C virus helicase/NTPase: an efficient expression system and new inhibitors

Content Provider	Springer Nature Link
Author	Mukovnya, A. V. Tunitskaya, V. L. Khandazhinskaya, A. L. Golubeva, N. A. Zakirova, N. F. Ivav, A. V. Kukhava, M. K. Kochetkov, S. N.
Copyright Year	2008
Abstract	A method has been developed for obtaining a full-length protein NS3 of hepatitis C virus with the yield of 6.5 mg/liter of cell culture, and conditions for measuring its NTPase and helicase activities have been optimized. The helicase reaction can proceed in two modes depending on the enzyme and substrate concentration ratio: it can be non-catalytic in the case of enzyme excess and catalytic in the case of tenfold substrate excess. In the latter case, helicase activity is coupled with NTPase and is stimulated by ATP. A number of NTP and inorganic pyrophosphate analogs were studied as substrates and/or inhibitors of NS3 NTPase activity, and it was found that the structure of nucleic base and ribose fragment of NTP molecule has a slight effect on its inhibitory (substrate) properties. Among the nucleotide derivatives, the most efficient inhibitor of NTPase activity is 2′-deoxythymidine 5′-phosphoryl-β,γ-hypophosphate, and among pyrophosphate analogs imidodiphosphate exhibited maximal inhibitory activity. These compounds were studied as inhibitors of the helicase reaction, and it was shown that imidodiphosphate efficiently inhibited the ATP-dependent helicase reaction and had almost no effect on the ATP-independent duplex unwinding. However, the inhibitory effect of 2′-deoxythymidine 5′-phosphorylβ,γ-hypophosphate was insignificant in both cases, which is due to the possibility of helicase activation by this ATP analog.
Starting Page	660
Ending Page	668
Page Count	9
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	73
Issue Number	6
e-ISSN	16083040
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2008-07-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	hepatitis C virus protein NS3 NTPase helicase NTP analogs inorganic pyrophosphate analogs Biomedicine general Microbiology Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

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