NDLI: Activity of tissue factor in microparticles produced in vitro by endothelial cells, monocytes, granulocytes, and platelets

Content Provider	Springer Nature Link
Author	Khaspekova, S. G. Antova, O. A. Shustova, O. N. Yakushkin, V. V. Golubeva, N. V. Titaeva, E. V. Dobrovolsky, A. B. Mazurov, A. V.
Copyright Year	2016
Abstract	Activity of tissue factor (TF) in membrane microparticles (MPs) produced in vitro by endothelial cells (ECs), monocytes, THP-1 monocytic cells, granulocytes, and platelets was investigated. ECs were isolated from human umbilical vein, and monocytes, granulocytes, and platelets–from the blood of healthy donors. ECs, monocytes, and THP-1 cells were activated by bacterial lipopolysaccharide, granulocytes–by lipopolysaccharide or phorbol myristate acetate, and platelets - by SFLLRN, thrombin receptor-activating peptide. MPs were sedimented from the culture medium or supernatant of activated cells at 20,000g for 30 min. Coagulation activity of MPs was analyzed in a modified recalcification assay by assessing their effects on coagulation of donor plasma depleted of endogenous MPs (by centrifuging at 20,000g for 90 min). MPs from all cell types accelerated plasma coagulation. Antibodies blocking TF activity prolonged coagulation lagphase in the presence of MPs from ECs, monocytes, and THP-1 cells (by 2.7-, 2.0-, and 1.8-fold, respectively), but did not influence coagulation in the presence of MPs from granulocytes and platelets. In accordance with these data, TF activity measured by its ability to activate factor X was found in MPs from ECs, monocytes, and THP-1 cells, but not in MPs from granulocytes and platelets. The data obtained indicate that active TF is present in MPs produced in vitro by ECs, monocytes, and THP-1 cells, but not in MPs derived from granulocytes and platelets.
Starting Page	114
Ending Page	121
Page Count	8
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	81
Issue Number	2
e-ISSN	16083040
Language	English
Publisher	Pleiades Publishing
Publisher Date	2016-02-16
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	membrane microparticles coagulation tissue factor endothelial cells monocytes granulocytes platelets Biochemistry Bioorganic Chemistry Microbiology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

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