NDLI: Inactivation of genes encoding superoxide dismutase modifies yeast response to S-nitrosoglutathione-induced stress

Content Provider	Springer Nature Link
Author	Lushchak, O. V. Nykorak, N. Z. Ohdate, T. Iue, Y. Lushchak, V. I.
Copyright Year	2009
Abstract	Antioxidant enzymes can modify cell response to nitrosative stress induced, for example, by nitric oxide or compounds decomposing with its formation. Therefore, we investigated the effects of S-nitrosoglutathione (GSNO) on cell survival, activity of antioxidant enzymes, and concentrations of reduced and oxidized glutathione in parental and isogenic strains defective in Cu,Zn- or Mn-superoxide dismutases (Cu,Zn-SOD and Mn-SOD, respectively), or in both of them. Stress was induced by incubation of the yeast with 1–20 mM GSNO. The strains used demonstrated different sensitivity to GSNO. A Cu,Zn-SOD-defective strain survived the stress better than the parental strain, while the double mutant was the most sensitive to GSNO. The ·NO-donor at low concentrations (1–5 mM) increased SOD activity, but its high concentrations (10 and 20 mM) decreased it. The activity of catalase in all strains was enhanced by GSNO. Inhibition of protein synthesis by cycloheximide did not prevent the activation of SOD, but it prevented the activation of catalase. These facts suggest that SOD was activated at a posttranslational level and catalase activity was enhanced via de novo synthesis. A GSNO-induced increase in oxidized glutathione level in the studied yeast strains might account for cell killing by GSNO due to the development of oxidative/nitrosative stress.
Starting Page	445
Ending Page	451
Page Count	7
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	74
Issue Number	4
e-ISSN	16083040
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2009-04-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	yeast superoxide dismutase S-nitrosoglutathione nitric oxide markers of oxidative stress Biomedicine general Microbiology Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Construction of a fusion enzyme exhibiting superoxide dismutase and peroxidase activity

Role of thiamine thiol form in nitric oxide metabolism

Oxidative stress in yeast

Catalase modifies yeast Saccharomyces cerevisiae response towards S-nitrosoglutathione-induced stress.

Involvement of soxRS Regulon in Response of Escherichia coli to Oxidative Stress Induced by Hydrogen Peroxide

Mitochondrial metabolism of reactive oxygen species

Purification and characterization of Fe-containing superoxide dismutase from Methanobrevibacter arboriphilus strain AZ

Gene expression characteristics and regulation mechanisms of superoxide dismutase and its physiological roles in plants under stress

Bacterial NO synthases

Inactivation of genes encoding superoxide dismutase modifies yeast response to S-nitrosoglutathione-induced stress

Similar Documents

Construction of a fusion enzyme exhibiting superoxide dismutase and peroxidase activity

Role of thiamine thiol form in nitric oxide metabolism

Oxidative stress in yeast

Catalase modifies yeast Saccharomyces cerevisiae response towards S-nitrosoglutathione-induced stress.

Involvement of soxRS Regulon in Response of Escherichia coli to Oxidative Stress Induced by Hydrogen Peroxide

Mitochondrial metabolism of reactive oxygen species

Purification and characterization of Fe-containing superoxide dismutase from Methanobrevibacter arboriphilus strain AZ

Gene expression characteristics and regulation mechanisms of superoxide dismutase and its physiological roles in plants under stress

Bacterial NO synthases

Inactivation of genes encoding superoxide dismutase modifies yeast response to S-nitrosoglutathione-induced stress