NDLI: Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings

Content Provider	Springer Nature Link
Author	Kumari, G. J. Reddy, A. M. Naik, S. T. Kumar, S. G. Prasanthi, J. Sriranganayakulu, G. Reddy, P. C. Sudhakar, Chinta
Copyright Year	2006
Abstract	Protein pattern, ammonia content, glutamine synthetase activity, lipid peroxidation, superoxide dismutase, catalase, peroxidase and peroxidase isoforms were studied in the leaves and roots of 7-d-old peanut (Arachis hypogaea L. cv. JL-24) seedlings treated by 25, 100 and 250 μM jasmonic acid (JA). SDS-PAGE protein profile of leaves and roots after JA application showed a significant increase in 18, 21, 30, 45, 47 and 97.4 kDa proteins and significant decrease in 22 and 36 kDa proteins. Pathogenesis related PR-18 was specific in leaves at 250 μM JA and PR-21 have cross reacted differently with 21 and 30 kDa proteins in leaves and roots treated by all JA concentrations. Further, the immunoblot analysis with glutamine synthetase, GS-45 antibodies revealed a specific cross reaction with 45 and 47 kDa proteins of both control and JA treated leaves, however, higher at 100 and 250 μM JA treated leaves than control ones. Further, the malondialdehyde (MDA) content significantly increased in leaves and roots treated with JA, indicated membrane damage with JA treatments that led to the generation of peroxidation products. The peroxidase isozymic pattern showed two specific isoforms. Besides, the activities of SOD and catalase were significantly elevated in JA treated leaves.
Starting Page	219
Ending Page	226
Page Count	8
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	50
Issue Number	2
e-ISSN	15738264
Language	English
Publisher	Kluwer Academic Publishers-Consultants Bureau
Publisher Date	2006-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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