NDLI: Sodium nitroprusside modulates gene expression involved in glutathione synthesis in Zea mays leaves

Content Provider	Springer Nature Link
Author	Mello, C. Souza Hermes, V. S. Guerra, M. P. Arisi, A. C. M.
Copyright Year	2012
Abstract	To investigate a possible involvement of nitric oxide in gene regulation of glutathione and flavonoid synthesis pathways, maize seedlings were treated with sodium nitroprusside (SNP), a NO donor, and apocynin (APO), an inducer of NO production. After 12-h treatment, the transcripts of γ-glutamylcysteine synthetase (γ-ecs), glutathione synthetase (gsh-s), chalcone synthase (chs), phenylalanine ammonia lyase (pal.1), myb-related protein P (P1) and actin 1 (act) genes were quantified in maize leaves by real time PCR, using α-tubulin as standard transcript. The level of γ-ecs and gsh-s transcripts in maize leaves were increased 9-fold and 12-fold, respectively, following SNP treatment, while after APO treatment, those transcripts were not significantly different from control plants. SNP-treated maize leaves did not show significant changes in pal.1 and chs expression. NO content in maize leaves was increased in SNP and APO treated plants in comparison to control plants. In conclusion, our experiments suggested that genes involved in glutathione synthesis could be modulated by SNP in maize leaves. On the other hand, APO had no effect on γ-ecs and gshs gene expression.
Starting Page	383
Ending Page	388
Page Count	6
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	56
Issue Number	2
e-ISSN	15738264
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-03-03
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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