NDLI: Activity of β-glucuronidase in Root Tips of Different Types of Transgenic Sugar Beet Plants

Content Provider	Springer Nature Link
Author	Vitha, S. Phillips, J.P. Gartland, J.S. Gartland, K.M.A. Beneš, K. Elliott, M.C.
Copyright Year	1997
Abstract	Expression of the β-glucuronidase (GUS) reporter gene driven by the CaMV 35S, rolC, nos and mas promoters was assessed in the tips of 12 independent clones of transgenic sugar beet (Beta vulgaris) roots. Three questions were addressed: 1) expression pattern specific for a given promoter, 2) expression pattern variability, and 3) relationship between gene expression and cell differentiation. Characteristic patterns of tissue-specific expression were distinguished for each promoter. Striking differences, however, were found between some clones, bearing the same construct. Statistical analysis of the pattern variability proved that the variability is significantly lower within the construct than between constructs. rolC-GUS clones exhibited the lowest and CaMV 35S clones the highest pattern variability. Comparisons between the four promoters showed consistent GUS activity in areas playing a key role in tissue determination (the elongation zone) where cells switch from frequent mitosis and mostly isodiametrical growth, typical for the promeristem, to rapid elongation and differentiation. All of the clones were highly GUS-positive in the elongation zone of stele. Activity was commonly localised in the stele of the maturation zone for CaMV 35S, rolC and mas-GUS clones. CaMV 35S-GUS clones were highly active in the promeristem.
Starting Page	531
Ending Page	541
Page Count	11
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	40
Issue Number	4
e-ISSN	15738264
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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