NDLI: Molecular cloning and characterization of a novel stress responsive gene in alfalfa

Content Provider	Springer Nature Link
Author	Long, R. Yang, Q. Kang, J. Chao, Y. Wang, P. Wu, M. Qin, Z. Sun, Y.
Copyright Year	2012
Abstract	A suppression subtraction hybridization (SSH) cDNA library of alfalfa (Medicago sativa L.) cv. Zhongmu NO.1 had been constructed to identify differentially expressed genes under stress. Based on the sequence of a 460 bp expressed sequence tags (ESTs), a cDNA of 1652 bp was cloned by rapid amplification of cDNA ends (RACE) method. This gene (MsPBL) was predicted to encode a 434-amino-acid protein, which contained a Phox and Bem1 (PB1) domain. PB1 domain is a functional domain comprising about 80 amino acid residues, which exists in many signal transduction proteins and mediates dimerization in the proteins. PB1 domain is mostly involved in two cell signal transduction pathways: MAPK and NF-KB. When fused to the green fluorescent protein, we found MsPBL localization in the nucleus of onion (Allium cepa L.) epidermal cells. The transcripts of MsPBL rose significantly when alfalfa was treated with 300 mM NaCl, 0.1 mM ABA, and 20 % polyethylene glycol (PEG-6000). These results indicated that MsPBL may be functional within the nucleus as a signal transduction protein to allow alfalfa to rapidly respond to the environmental stress signals.
Starting Page	43
Ending Page	49
Page Count	7
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	56
Issue Number	1
e-ISSN	15738264
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-01-21
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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