NDLI: In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli

Content Provider	Springer Nature Link
Author	Agrawal, V. Sardar, P. R.
Copyright Year	2006
Abstract	High efficiency shoot regeneration was achieved through leaflet and cotyledon derived calli in Cassia angustifolia - an important medicinal plant. Dark brown compact callus was induced at the cut ends of the explants on Murashige and Skoog's (MS) medium augmented with 1 µM N6-benzyladenine (BA) + 1 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Such callus pieces on transfer to cytokinins (BA or kinetin) supplemented medium differentiated shoots within 10 – 15 d. Of the two cytokinins, 5 µM BA was optimum for eliciting morphogenic response in 83.33 and 70.83 % cultures with an average of 4.16 ± 0.47 and 3.70 ± 0.56 shoots in cotyledon and leaflet derived calli, respectively. The addition of 0.5 µM α-naphthaleneacetic acid (NAA) to MS + 5 µM BA further elevated the maximum average number of shoots to 12.08 ± 1.04 and 5.37 ± 0.52 for cotyledon and leaflet calli, respectively. The excised shoots were transferred to a rooting medium containing either IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) or NAA. Nearly 95 % shoots developed an average of 5.4 ± 0.41 roots on half strength MS medium supplemented with 10 µM IBA.
Starting Page	118
Ending Page	122
Page Count	5
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	50
Issue Number	1
e-ISSN	15738264
Language	English
Publisher	Kluwer Academic Publishers-Consultants Bureau
Publisher Date	2006-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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