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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kant, P. Kant, S. Jain, R. K. Chaudhury, V. K. |
| Copyright Year | 2007 |
| Abstract | The Agrobacterium-mediated transformation was done in rice (Oryza sativa L. var. indica) cv. HKR126 and elite cross-bred cv. Pusa Basmati1 (PB1), using strain LBA4404 containing pCAMBIA1300 cloned with gene cassettes; potato proteinase inhibitor and Bacillus thuringiensis endotoxin (plasmid JDW53) or mannitol-1-phosphate dehydrogenase (plasmid RKJ108). Co-cultivation with scutellar-calli derived from mature seeds showed stable and highly efficient transformation. In cvs. HKR126 and PB1, 35 % and 41 % of hygromycin resistant calli were obtained. The transformation efficiency in PB1 (22.0 %) was much higher than in HKR126 (12.5 %). Similarly, PB1 had higher plant regeneration efficiency than HKR126. The shoots regenerated per callus were, 3–4 in HKR126 and 5–6 in PB1. The transformation efficiency with pRKJ108 (18.6 %) was higher than pJDW53 (15.9 %). Polymerase chain reaction (PCR) analysis showed the presence of transgenes in regenerated transgenic plants of both cultivars. |
| Starting Page | 61 |
| Ending Page | 68 |
| Page Count | 8 |
| File Format | |
| ISSN | 00063134 |
| Journal | Biologia Plantarum |
| Volume Number | 51 |
| Issue Number | 1 |
| e-ISSN | 15738264 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2007-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | Subscribed |
| Subject Keyword | Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Horticulture |
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