NDLI: Effects of dietary methylmercury on the zebrafish brain: histological, mitochondrial, and gene transcription analyses

Content Provider	Springer Nature Link
Author	Cambier, Sébastien Gonzalez, Patrice Mesmer Dudons, Nathalie Brèthes, Daniel Fujimura, Masatake Bourdineaud, Jean Paul
Copyright Year	2011
Abstract	The neurotoxic compound methylmercury (MeHg) is a commonly encountered pollutant in the environment, and constitutes a hazard for wildlife and human health through fish consumption. To study the neurotoxic impact of MeHg on piscivorous fish, we contaminated the model fish species Danio rerio for 25 and 50 days with food containing 13.5 μg/g dry weight (dw) of MeHg (0.6 μg MeHg/fish/day), an environmentally relevant dose leading to brain mercury concentrations of 30 ± 4 μg of Hg g−1 (dw) after 25 days of exposure and 46 ± 7 μg of Hg g−1 (dw) after 50 days. Brain mitochondrial respiration was not modified by exposure to MeHg, contrary to what happens in skeletal muscles. A 6-fold increase in the expression of the sdh gene encoding the succinate dehydrogenase Fe/S protein subunit was detected in the contaminated brain after 50 days of exposure. An up regulation of 3 genes, atp2b3a, atp2b3b, and slc8a2b, encoding for calcium transporters was noticed after 25 days of exposure but the atp2b3a and atp2b3b were repressed and the slc8a2b gene expression returned to its basal level after 50 days, suggesting a perturbation of calcium homeostasis. After 50 days, we detected the up regulation of glial fibrillary acidic protein and glutathione S-transferase genes (gfap and gst), along with a repression of the glutathione peroxidase gene gpx1. These results match well with a MeHg-induced onset of oxidative stress and inflammation. A transmission electron microscopic observation confirmed an impairment of the optical tectum integrity, with a decrease of the nucleal area in contaminated granular cells compared to control cells, and a lower density of cells in the contaminated tissue. A potential functional significance of such changes observed in optical tectum when considering wild fish contaminated in their natural habitat might be an impaired vision and therefore a lowered adaptability to their environment.
Starting Page	165
Ending Page	180
Page Count	16
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	25
Issue Number	1
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Methylmercury Dietary exposure Zebrafish Bioenergetics Brain Microbiology Pharmacology/Toxicology Plant Physiology Medicine/Public Health Cell Biology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

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