NDLI: Phosphate relieves chromium toxicity in Arabidopsis thaliana plants by interfering with chromate uptake

Content Provider	Springer Nature Link
Author	López Bucio, José Hernández Madrigal, Fátima Cervantes, Carlos Ortiz Castro, Randy Carreón Abud, Yazmín Martínez Trujillo, Miguel
Copyright Year	2014
Abstract	Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. Mineral nutrients such as phosphate (Pi), sulfate and nitrate have been reported to attenuate Cr(VI) toxicity, but the underlying mechanisms remain to be clarified. Here, we show that chromate activates the expression of low-Pi inducible reporter genes AtPT1 and AtPT2 in Arabidopsis thaliana transgenic seedlings. Primary-root growth was inhibited by 60 % in AtPT2::uidA-expressing seedlings upon exposure to 140-μM Cr(VI). However, increasing the Pi and sulfate supply to the seedlings that were experiencing Cr(VI) toxicity completely and partially restored the root growth, respectively. This effect correlated with the Cr(VI)-induced AtPT2::uidA expression being completely reversed by addition of Pi. To evaluate whether the nutrient supply may affect the endogenous level of Cr in plants grown under toxic Cr(VI) levels, the contents of Cr were measured (by ICP-MS analyses) in seedlings treated with Cr and with or without Pi, sulfate or nitrate. It was found that Cr accumulation increases tenfold in plants treated with 140-μM Cr(VI) without modifying the phosphorus concentration in the plant. In contrast, the supply of Pi specifically decreased the Cr content to levels similar to those found in seedlings grown in medium without chromate. Taken together, these results show that in A. thaliana seedlings the uptake of Cr(VI) is reduced by Pi. Moreover, our data indicate that Pi and sulfate supplements may be useful in strategies for handling Cr-contaminated soils.
Starting Page	363
Ending Page	370
Page Count	8
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	27
Issue Number	2
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-02-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis thaliana Chromate Phosphate Sulfate Root development Metal uptake Biochemistry Pharmacology/Toxicology Microbiology Cell Biology Plant Physiology Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Chromate induces adventitious root formation via auxin signalling and SOLITARY-ROOT/IAA14 gene function in Arabidopsis thaliana

Synechococcus elongatus PCC 7942 is more tolerant to chromate as compared to Synechocystis sp. PCC 6803

Impact of heavy metal stresses on the growth and auxin homeostasis of Arabidopsis seedlings

Physiological metal uptake by Nostoc punctiforme

Chelation of chromium(VI) by combining deferasirox and deferiprone in rats

Mercury toxicity, molecular response and tolerance in higher plants

Toxicity and tolerance of aluminum in plants: tailoring plants to suit to acid soils

Nicotianamine is a major player in plant Zn homeostasis

Impact of hexavalent chromium on mammalian cell bioenergetics: phenotypic changes, molecular basis and potential relevance to chromate-induced lung cancer

Phosphate relieves chromium toxicity in Arabidopsis thaliana plants by interfering with chromate uptake

Similar Documents

Chromate induces adventitious root formation via auxin signalling and SOLITARY-ROOT/IAA14 gene function in Arabidopsis thaliana

Synechococcus elongatus PCC 7942 is more tolerant to chromate as compared to Synechocystis sp. PCC 6803

Impact of heavy metal stresses on the growth and auxin homeostasis of Arabidopsis seedlings

Physiological metal uptake by Nostoc punctiforme

Chelation of chromium(VI) by combining deferasirox and deferiprone in rats

Mercury toxicity, molecular response and tolerance in higher plants

Toxicity and tolerance of aluminum in plants: tailoring plants to suit to acid soils

Nicotianamine is a major player in plant Zn homeostasis

Impact of hexavalent chromium on mammalian cell bioenergetics: phenotypic changes, molecular basis and potential relevance to chromate-induced lung cancer

Phosphate relieves chromium toxicity in Arabidopsis thaliana plants by interfering with chromate uptake