NDLI: Release of intracellular Zn2+ in cultured neurons after brief exposure to low concentrations of exogenous nitric oxide

Content Provider	Springer Nature Link
Author	Lin, Wei Mohandas, Bhavana Fontaine, Charles P. Colvin, Robert A.
Copyright Year	2007
Abstract	Several studies have shown intracellular Zn2+ release and concomitant cell death after prolonged exposure to exogenous NO. In the present study, we investigated whether cortical neurons briefly exposured to exogenous NO would demonstrate similar levels of intracellular Zn2+ release and subsequent cell death. Cortical neurons were loaded with the Zn2+ selective fluorophore FluoZin-3 and treated with various concentrations of the NO generator, spermine NONOate. Fluorescence microscopy was used to detect and quantify intracellular Zn2+ levels. Concomitant EDTA perfusion was used to eliminate potential effects of extracellular Zn2+. Neurons were perfused with the heavy metal chelator TPEN to selectively eliminate Zn2+ induced fluorescence changes. A significant increase of intracellular fluorescence was detected during a 5 min perfusion with spermine NONOate. The increase in intracellular Zn2+ release appeared to peak at 1 μM spermine NONOate (123.8 ± 28.5%, increase above control n = 20, P < 0.001). Further increases in spermine NONOate levels as high as 1 mM failed to further increase detectable intracellular Zn2+ levels. The NO scavenger hemoglobin blocked the effects of spermine NONOate and the inactive analog of the spermine NONOate, spermine, was without effect. No evidence of cell death induced by any of the brief treatments with exogenous NO was observed; only prolonged incubation with much larger amounts of exogenous NO resulted in significant cell death. These data suggest that in vivo release of NO may cause elevations of intracellular Zn2+ in cortical neurons. The possibility that release of intracellular Zn2+ in response to NO could play a role in intracellular signaling is discussed.
Starting Page	891
Ending Page	901
Page Count	11
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	20
Issue Number	6
e-ISSN	15728773
Language	English
Publisher	Springer US
Publisher Date	2007-02-06
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Intracellular Zn2+ FluoZin-3 culture Metallothionein Physical Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

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