NDLI: The chromate resistance phenotype of some yeast mutants correlates with a lower level of Cr(V)-species generated in the extra-cellular medium

Content Provider	Springer Nature Link
Author	Ksheminska, Helena Honchar, Taras Usatenko, Yuriy Gayda, Galina Gonchar, Mykhailo
Copyright Year	2010
Abstract	The paper describes the selection of chromate-resistant mutants of the yeast Pichia guilliermondii with a higher chromate-reducing activity and reports the EPR-study of Cr(V)-generation in the extra-cellular medium during the reduction of chromate by the yeast culture. It is shown that the reduction of chromate to Cr(III) species runs through the extra-cellular generation of Cr(V)-intermediate(s), thus supporting the assumption about the existence of an extra-cellular pathway of Cr(VI)-reduction. Furthermore, it is demonstrated that the chromate-resistance phenotype of tested mutants correlates with a lower stationary level of Cr(V)-species in the medium. It is thus suggested that isolated mutants can be used as sources of Cr(III)-biocomplexes due to their ability to effectively reduce chromate to Cr(III)-chelates with potential pharmacological applications.
Starting Page	633
Ending Page	642
Page Count	10
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	23
Issue Number	4
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chromate reduction Yeast Chromium(V) Chromate-resistant mutants Chromium(III)-biocomplexes Physical Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

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