NDLI: BjHO-1 is involved in the detoxification of heavy metal in India mustard (Brassica juncea)

Content Provider	Springer Nature Link
Author	Li, Hua Jiang, Ming Che, Li Ling Nie, Li Yang, Zhi Min
Copyright Year	2012
Abstract	Heme oxygenase-1 (HO-1) is a stress-responsive gene coding for an enzyme catalyzing the catabolism of heme to yield biliverdin IXα, carbon monoxide (CO) and iron. However, its biological role in regulating metal homeostasis, particularly the tolerance to toxic heavy metals is poorly understood. In this study, a novel gene encoding a Brassica juncea heme oxygenase-1 (designated as BjHO-1) was cloned and functionally identified. Spatial expression of BjHO-1 showed that it was differentially expressed in cotyledon, hypocotyl, leaf and root. BjHO-1 was found to be induced significantly by heavy metal Hg. To understand whether BjHO-1 is able to regulate plant tolerance to Hg, we constructed transgenic B. juncea plants overexpressing HO-1, and showed that 35S::BjHO1 plants confer the plant resistance to Hg toxicity by improving plant dry mass, reducing Hg accumulation, and attenuating Hg-induced oxidative stress. We further cloned a 1,099 bp promoter sequence upstream of BjHO-1 using genome walking approach. Multiple stress-responsive elements were detected in the BjHO-1 promoter regions. The promoter can be activated by Zn, Cd, Hg and Pb exposure. Our results indicate that up-regulation of BjHO-1 is beneficial for limiting the uptake or accumulation of heavy metals into plants. This work also provides a new example for molecular breeding designed for plants that do not accumulate or minimizing accumulation of toxic trace metals growing on heavy metal-contaminated soils.
Starting Page	1269
Ending Page	1279
Page Count	11
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	25
Issue Number	6
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-10-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica juncea BjHO-1 Heavy metals Accumulation Detoxification Pharmacology/Toxicology Microbiology Plant Physiology Cell Biology Biochemistry Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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