NDLI: Heterogenous distribution of ferroportin-containing neurons in mouse brain

Content Provider	Springer Nature Link
Author	Boserup, Michael W. Lichota, Jacek Haile, David Moos, Torben
Copyright Year	2011
Abstract	Iron is crucial for a variety of cellular functions in neuronal cells. Neuronal iron uptake is reflected in a robust and consistent expression of transferrin receptors and divalent metal transporter 1 (DMT 1). Conversely, the mechanisms by which neurons neutralize and possibly excrete iron are less clear. Studies indicate that neurons express ferroportin which could reflect a mechanism for iron export. We mapped the distribution of ferroportin in the adult mouse brain using an antibody prepared from a peptide representing amino acid sequences 223–303 of mouse ferroportin. The antibody specifically detected ferroportin in brain homogenates, whereas homogenates of cultured endothelial cells were devoid of immunoreactivity. In brain sections, ferroportin was confined to neuronal cell bodies and peripheral processes of cerebral cortex, hippocampus, thalamus, brain stem, and cerebellum. In brain stem ferroportin-labeling was particularly high in neurons of cranial nerve nuclei and reticular formation. Ferroportin was hardly detectable in striatum, pallidum, or hypothalamus. Among non-neuronal cells, ferroportin was detected in oligodendrocytes and choroid plexus epithelial cells. A comparison with previous studies on the distribution of transferrin receptors in neurons shows that many neuronal pools coincide with those expressing ferroportin. The data therefore indicate that neuronal iron homeostasis consists of a delicate balance between transferrin receptor-mediated uptake of iron-transferrin and ferroportin-related iron excretion. The findings also suggest a particular high turnover of iron in neuronal regions, such as habenula, hippocampus, reticular formation and cerebellum, as several neurons in these regions exhibit a prominent co-expression of transferrin receptors and ferroportin.
Starting Page	357
Ending Page	375
Page Count	19
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	24
Issue Number	2
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Blood–brain barrier Iron Neurodegeneration Oxidative stress qPCR Transferrin Biochemistry Physical Chemistry
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Metals ions and neurodegeneration

Mechanistic analysis of iron accumulation by endothelial cells of the BBB

Non-transferrin bound iron, cytokine activation and intracellular reactive oxygen species generation in hemodialysis patients receiving intravenous iron dextran or iron sucrose

Age-related changes in iron homeostasis in mouse ferroxidase mutants

Intestinal iron absorption during suckling in mammals

Iron-induced oxidative stress in haemodialysis patients: a pilot study on the impact of diabetes

Non-transferrin-bound iron in plasma following administration of oral iron drugs

Cadmium and mercury cause an oxidative stress-induced endothelial dysfunction

A structural comparison of human serum transferrin and human lactoferrin

Heterogenous distribution of ferroportin-containing neurons in mouse brain

Similar Documents

Metals ions and neurodegeneration

Mechanistic analysis of iron accumulation by endothelial cells of the BBB

Non-transferrin bound iron, cytokine activation and intracellular reactive oxygen species generation in hemodialysis patients receiving intravenous iron dextran or iron sucrose

Age-related changes in iron homeostasis in mouse ferroxidase mutants

Intestinal iron absorption during suckling in mammals

Iron-induced oxidative stress in haemodialysis patients: a pilot study on the impact of diabetes

Non-transferrin-bound iron in plasma following administration of oral iron drugs

Cadmium and mercury cause an oxidative stress-induced endothelial dysfunction

A structural comparison of human serum transferrin and human lactoferrin

Heterogenous distribution of ferroportin-containing neurons in mouse brain