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| Content Provider | Springer Nature Link |
|---|---|
| Author | Barnes, H.H. Ishimaru, Carol A. |
| Copyright Year | 1999 |
| Abstract | Catechols are co-planar cis-diols known to form stable, isolable complexes with borate under weakly basic conditions. We exploited this chemistry and developed a boronate affinity chromatography for isolating catechol siderophores. The method was applied to the isolation of chrysobactin, enterobactin, and an unknown catechol siderophore produce by Erwinia carotovora subsp. carotovora W3C105. Yields of chrysobactin and enterobactin purified by boronate affinity chromatography were at least two-fold greater than those achieved through alternate methods. The unknown catechol produced by E. carotovora subsp. carotovora W3C105 was isolated by boronate affinity chromatography and shown to be identical to chrysobactin. Boronate affinity chromatography enabled separation of catechol from its rust-colored decomposition products, and simultaneous isolation of catechol and hydroxamate siderophores. Boronate affinity chromatography is a rapid and efficient method for purifying catechol siderophores from bacterial culture supernatants |
| Starting Page | 83 |
| Ending Page | 87 |
| Page Count | 5 |
| File Format | |
| ISSN | 09660844 |
| Journal | BioMetals |
| Volume Number | 12 |
| Issue Number | 1 |
| e-ISSN | 15728773 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 1999-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Physical Chemistry Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences |
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