NDLI: Ferrous iron chelating property of low-molecular weight succinoglycans isolated from Sinorhizobium meliloti

Content Provider	Springer Nature Link
Author	Cho, Eunae Choi, Jae Min Kim, Hwanhee Tahir, Muhammad Nazir Choi, Youngjin Jung, Seunho
Copyright Year	2013
Abstract	Iron is an essential nutrient for nitrogen-fixing legume root nodules, and the chelation of ferrous iron plays an important role in the mobility and availability of iron to the legume. In the present study, we investigated the iron-binding properties of low-molecular weight succinoglycans isolated from the nitrogen-fixing bacterium, Sinorhizobium meliloti. The low-molecular weight succinoglycans comprising three monomers (M1–M3), four dimers (D1–D4), and six trimers (T1–T6) of the succinoglycan repeating unit were purified by various chromatographic techniques. Interestingly, the colorimetric ferrozine method showed that the succinoglycans T6, M3, and D3 demonstrated a ferrous iron chelating ability of 83, 63, and 38 % per mg, respectively. The individual binding constants were determined as 43703, 2313, and 760 M−1 for succinoglycans T6, M3, and D3 using ultraviolet–visible spectroscopy. The complexation of succinoglycan and ferrous iron can cause structural changes, which were analyzed by circular dichroism spectroscopy. Furthermore, the complex could provide antioxidant activity through an anti-Fenton reaction. These results demonstrate that the low-molecular weight succinoglycans can effectively modulate iron biochemistry as a novel ferrous iron-acquisition system of S. meliloti.
Starting Page	321
Ending Page	328
Page Count	8
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	26
Issue Number	2
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-02-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Low-molecular weight succinoglycans Sinorhizobium meliloti Ferrous iron Chelating ability Fenton reaction Biochemistry Pharmacology/Toxicology Microbiology Cell Biology Plant Physiology Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

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