NDLI: A novel n-alkane-degrading bacterium as a minor member of p-xylene-degrading sulfate-reducing consortium

Content Provider	Springer Nature Link
Author	Higashioka, Yuriko Kojima, Hisaya Nakagawa, Tatsuri Sato, Shinya Fukui, Manabu
Copyright Year	2008
Abstract	A p-xylene-degrading, sulfate-reducing enrichment culture was characterized by analyzing the response of its members to changes in the available substrate. The culture was inoculated into media containing other substrates, resulting in the establishment of benzoate-, acetate-, and lactate-utilizing enrichment cultures. PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the enriched cultures targeting 16S rRNA genes showed quite simple band patterns. The predominant band from the benzoate-utilizing enrichment culture was identical to that from the original enrichment culture utilizing p-xylene. A single, dominant DGGE band was observed in common from the acetate- and lactate-utilizing enrichment cultures. A novel sulfate-reducing bacterium, strain PL12, was isolated from the lactate-utilizing enrichment culture. The 16S rRNA gene sequence of strain PL12 was identical to that of the dominant DGGE band in the acetate- and lactate-utilizing enrichment cultures and distinct from the dominant sequences in the original p-xylene-degrading and benzoate-utilizing enrichment cultures. Phylogenetic analysis of the 16S rRNA gene sequences showed that the isolate belonged to the family Desulfobacteraceae in the class Deltaproteobacteria. The isolated strain PL12 could utilize n-hexane and n-decane as substrates, but could not utilize benzoate, p-xylene and other aromatic hydrocarbons. These results suggest that the p-xylene degradation observed in the original enrichment culture was performed by the dominant bacterium corresponding to DGGE band pXy-K-13 (Nakagawa et al. 2008). The novel strain PL12 might have been utilizing metabolites of p-xylene.
Starting Page	383
Ending Page	390
Page Count	8
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	20
Issue Number	3
e-ISSN	15729729
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-11-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sulfate-reducing bacteria p-Xylene n-Alkane Waste Management/Waste Technology Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Terrestrial Pollution Geochemistry Soil Science & Conservation Microbiology
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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