NDLI: Isolation and characterization of Microbulbifer species 6532A degrading seaweed thalli to single cell detritus particles

Content Provider	Springer Nature Link
Author	Wakabayashi, Masayuki Sakatoku, Akihiro da, Fumio da, Miru Tanaka, Daisuke Nakamura, Shogo
Copyright Year	2011
Abstract	To reduce the volume of seaweed wastes and extract polysaccharides, seaweed-degrading bacteria were isolated from drifting macroalgae harvested along the coast of Toyama Bay, Japan. Sixty-four bacterial isolates were capable of degrading “Wakame” (Undaria pinnatifida) thallus fragments into single cell detritus (SCD) particles. Amongst these, strain 6532A was the most active degrader of thallus fragments, and was capable of degrading thallus fragments to SCD particles within a day. Although the sequence similarity of the 16S rRNA gene of strain 6532A was 100% similar to that of Microbulbifer elongatus JAMB-A7, several distinct differences were observed between strains, including motility, morphology, and utilization of d-arabinose and gelatin. Consequently, strain 6532A was classified as a new Microbulbifer strain, and was designated Microbulbifer sp. 6532A. Strain 6532A was capable of degrading both alginate and cellulose in the culture medium, zymogram analysis of which revealed the presence of multiple alginate lyases and cellulases. To the best of our knowledge, this is the first study to directly demonstrate the existence of these enzymes in Microbulbifer species. Shotgun cloning and sequencing of the alginate lyase gene in 6532A revealed a 1,074-bp open reading frame, which was designated algMsp. The reading frame encoded a PL family seven enzyme composed of 358 amino acids (38,181 Da). With a similarity of 74.2%, the deduced amino acid sequence was most similar to a Saccharophagus enzyme (alg 7C). These findings suggest that algMsp in strain 6532A is a novel alginate lyase gene.
Starting Page	93
Ending Page	105
Page Count	13
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	23
Issue Number	1
e-ISSN	15729729
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-17
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Seaweed Degradation Alginate lyase Cellulase Microbulbifer Soil Science & Conservation Microbiology Waste Management/Waste Technology Geochemistry Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Terrestrial Pollution
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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