NDLI: Purification and characterization of an efficient poultry feather degrading-protease from Myrothecium verrucaria

Content Provider	Springer Nature Link
Author	Moreira Gasparin, Fabiana G. Souza, Cristina G. Marques Costa, Andréa M. Alexandri, Ana Maria Bracht, Cissa K. Boer, Cinthia G. Peralta, Rosane M.
Copyright Year	2009
Abstract	The purpose of this work was to characterize an alkaline protease from the filamentous fungus Myrothecium verrucaria and to explore its capability to degrade native poultry feathers. The enzyme was purified to homogeneity using a single chromatographic step. Recovery was high, 62%, with a specific activity of 12,851.8 U/mg protein. The enzyme is a small monomeric protein with a molecular mass of 22 ± 1.5 kDa. It presented pH optimum of 8.3 and was stable over a broad pH range (5.0–12.0). The temperature optimum was 37°C, with thermal stability at temperatures up to 45°C. The enzyme presented an efficiency of 80.3% in the degradation of poultry feather meal, releasing amino acids and soluble peptides. It was able to hydrolyze β-keratin without necessity of chemical or enzymatic reduction of the disulphide bonds. Considering that, everyday, poultry-processing plants produce feathers as a waste products, this protease can be useful in biotechnological processes aiming to improve the transformation of poultry feathers through solubilization of β-keratin into usable peptides. Furthermore, it can also be useful in processes aiming to reduce the environmental pollution caused by the accumulation of feathers.
Starting Page	727
Ending Page	736
Page Count	10
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	20
Issue Number	5
e-ISSN	15729729
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-04-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biodegradation Keratinase Poultry feather Protein purification Submerged cultures Waste Management/Waste Technology Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Terrestrial Pollution Geochemistry Soil Science & Conservation Microbiology
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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