NDLI: Characterization and application of an optical sensor for quantification of dissolved O2 in shake-flasks

Content Provider	Springer Nature Link
Author	Wittmann, Christoph Kim, Hyung Min John, Gert Heinzle, Elmar
Copyright Year	2003
Abstract	On-line measurement of dissolved O2 in shake-flasks was realized via immobilized sensor spots containing a fluorophore with an O2-dependent luminescent decay time. An unaffected sensor signal during 80 autoclaving cycles suggests multi-usage of sensor equipped shake-flasks. The sensor had a response time of 6 s. Quantification of gas-liquid mass transfer revealed maximum kLa values of 150 h−1, from which maximum O2 transfer capacity of 33 mM h−1 was calculated. Liquid volume and shaking frequency have a strong influence on kLa. Exemplified by cultivations of Corynebacterium glutamicum the importance of shaking rate for O2 supply of bacterial cultures is shown. Sampling of microbial cultures with intermittent shaking of a few minutes can cause O2 limitation. Based on the results of this work a simple and straightforward tool is now available for accurate O2 sensing in shake-flasks, which are widely used in microbial cultivations.
Starting Page	377
Ending Page	380
Page Count	4
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	25
Issue Number	5
e-ISSN	15736776
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2003-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Organic Chemistry Biochemistry Microbiology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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