NDLI: Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase

Content Provider	Springer Nature Link
Author	Xue jun, Cao Xing yan, Wu Fonseca, Luis J.P. Cabral, Joaquim M.S. Marcos, João Carlos
Copyright Year	2004
Abstract	Bioconversion of penicillin G in PEG 20000/dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with an intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7% (w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U ml−1, pH 7.8, temperature 37 °C, reaction time 40 min. Five repeated batches could be performed in these conditions. Conversions ratios between 0.9–0.99 mol of 6-aminopenicillanic acid (6-APA) per mol of penicillin G, were obtained and volumetric productivity was 3.6–4.6 μmol min−1 ml−1. In addition the product 6-APA could be directly crystallized from the top phase with a purity of 96%.
Starting Page	97
Ending Page	101
Page Count	5
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	26
Issue Number	2
e-ISSN	15736776
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Organic Chemistry Biochemistry Microbiology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase